iGEM國際基因工程機(jī)器大賽輔導(dǎo)

?一個關(guān)于合成生物學(xué)的世界頂級賽事

為什么參加IGEM?

全球最高水平的全成生物學(xué)學(xué)術(shù)學(xué)術(shù)活動
最受美國名校青睞的學(xué)術(shù)學(xué)術(shù)活動之一
學(xué)術(shù)活動機(jī)制涵蓋生物、數(shù)學(xué)、計算機(jī)、機(jī)械電子工程設(shè)計等多學(xué)科
與世界各地名校隊伍同臺競技，全面提高綜合素質(zhì)，開拓國際視野
浙大、武大等頂尖雙一流高校教授全程指導(dǎo)
專職名校博士團(tuán)隊傾力帶隊指導(dǎo)

IGEM學(xué)術(shù)活動輔導(dǎo)

1.基礎(chǔ)教學(xué)

（1）科學(xué)思維與學(xué)術(shù)研究導(dǎo)論
（2）合成生物學(xué)及基理論基礎(chǔ)課程
（3）IGEM學(xué)術(shù)活動規(guī)則指導(dǎo)
（4）IGEM學(xué)術(shù)活動團(tuán)隊領(lǐng)導(dǎo)力課程（中學(xué)基因工程學(xué)術(shù)社團(tuán)指導(dǎo)）
（5）基因工程基礎(chǔ)實驗
（6）生物信息學(xué)習(xí)與數(shù)學(xué)建模

2.實驗指導(dǎo)

（1）命題討論與聚焦
（2）命題設(shè)計與實驗設(shè)計指導(dǎo)
（3）實驗操作指導(dǎo)
（4）數(shù)據(jù)處理與建模指導(dǎo)
（5）論文寫作指導(dǎo)
（6）學(xué)術(shù)答辯指導(dǎo)

3.社會活動
（1）課題的公眾宣傳
（2）社會調(diào)研（企業(yè)、社區(qū)）
（3）學(xué)者調(diào)研（教授、院士、醫(yī)生）
（4）與其他參賽隊的互助實驗
（5）贊助方案與眾籌指導(dǎo)

4.項目展示
（1）海報、PPT設(shè)計
（2）項目展示頁面（WIKI）設(shè)計
（3）演講與答辯技巧指導(dǎo)

參賽指導(dǎo)
（1）赴美參賽的后勤保障
（2）比賽臨場指導(dǎo)

iGEM參賽時間安排表

時間	內(nèi)容
1-2月	賽前冬令營集訓(xùn)
3月	每周末線上培訓(xùn)，講解近期論文和往屆優(yōu)秀項目。 組織1次線下周末活動，探討項目，撰寫可行性方案。 設(shè)計第一批生物磚。
4月	周末線上培訓(xùn)。文獻(xiàn)閱讀和討論。 組織1次周末實驗，確定項目，確定DNA parts，訂購引物。 進(jìn)行預(yù)實驗構(gòu)建第一批生物磚。
5月	每周末線上交流，文獻(xiàn)閱讀和討論。 查閱資料，完善項目背景。開展有關(guān)項目背景的human practice 組織1次周末實驗，測試實驗條件。
6月	每周末線上交流，文獻(xiàn)閱讀和討論。 暑假前，組織學(xué)生1-2次周末實驗，獲取數(shù)據(jù)。
7月	進(jìn)實驗室做實驗，獲取實驗數(shù)據(jù)。 進(jìn)行數(shù)學(xué)建模訓(xùn)練。
8月	進(jìn)實驗室做實驗，獲取實驗數(shù)據(jù)。 組織學(xué)生參與隊伍交流。
9月	協(xié)助學(xué)生整理實驗數(shù)據(jù)，完成wiki框架和背景內(nèi)容。 完成ppt草稿 進(jìn)行與項目展示相關(guān)的human practice
10月	每周線上交流。整理wiki，poster，訓(xùn)練ppt展示。 比賽前邀請優(yōu)秀的科學(xué)家評委，安排1-2次ppt答辯
11月	賽前準(zhǔn)備，赴美比賽

?申請條件

（1）高一、高二在校學(xué)生（每隊8-15名）
（2）具備良好的英文聽說讀寫能力
（3）具有團(tuán)隊溝通、協(xié)作與表達(dá)能力
（4）對研究性學(xué)習(xí)有著濃厚的興趣、希望培養(yǎng)自已獨立思考、分析與解決問題的能力
（5）有一定的實驗、美術(shù)設(shè)計、編程、建模、攝影、英文等基礎(chǔ)將成為加分項

相關(guān)課程費用

1.第一階段【國內(nèi)參賽指導(dǎo)（必選）】

費用：60000元（明細(xì)如下：）

（1）隊伍注冊費

（2）項目與實驗指導(dǎo)費

（3）場地設(shè)備使用費

（4）實驗試劑耗材費

（5）實驗水電費

2.第二階段【赴美參賽費（可以選擇不去美國，如果不去可以不需要繳納這部分費用）】

費用：40000元（明細(xì)如下：）

（1）個人入場費

（2）簽證費用

（3）往返美國機(jī)票

（4）決賽期間住宿費

（5）決賽期間市內(nèi)出行費用

（6）決賽期間保險費

（7）決賽期間指導(dǎo)費

（8）決賽期間管理費

報名及面試流程

1.學(xué)生填寫報名表。
2.導(dǎo)師對申請者進(jìn)行審核。通過審核后，學(xué)生需參加面試。
3.學(xué)生通過面試后簽訂科研指導(dǎo)協(xié)議，并支付參賽輔導(dǎo)費、報名費、實驗室使用費、海外參賽費。
4.付費后，學(xué)員將收到錄取通知、實驗室報到通知，課程計劃以及預(yù)習(xí)資料包。

2018-2019IGEM獲獎作品部分展示（共9隊）

1.? 隊名：Great Bay China

項目名稱：microbial Compartmentalization AssisTed Nepetalactol Ingredient Production

獎項：

（1）Grand Prize Winner
（2）Best Wiki
（3）Best Poster
（4）Best Model
（5）Best New Composite Part
（6）Best Part Collection
（7）Best Product Design
（8）Best New Basic Part

摘要/項目介紹：

Nepetalactone is the active ingredient in catnip, a feline attractant, and a potential green pesticide. It has a common precursor, nepetalactol, with monoterpene indole alkaloids (MIAs) which is a group of plant-derived compounds of great therapeutic value, such as vincristine (an anti-cancer drug). We aim to synthesize nepetalactol through a mutualistic division of labour between E. coli and yeast. Besides, we design, characterize, and use a library of transcription activator-like effectors (TALE)stabilized promoters to regulate the heterologous gene expression in E. coli. Our applied design conceives the future application of nepetalactone on stray cat control, which we consider as an opportunity for public engagement and education.

2. 隊名：GreatBay_SZ

項目名稱：SPIDERMAN_SPIDroin EngineeRing with chroMoprotein And Natural dye

獎項：

（1）Grand Prize Winner;
（2）Best Education and Public Engagement;
（3）Best Hardware;
（4）Best Integrated Human Practices;
（5）Best Measuremen;
（6）Best Model;
（7）Best New Basic Part;
（8）Best New Composite Part;
（9）Best Part Collection;
（10）Best Poster;
（11）Best Presentation;
（12）Best Wiki;
（13）iGEMers' Prize

摘要/項目介紹：

Spider silk serves as a new material with superior properties that can be applied in medication, cloth, and aerospace fields. However, spider breeding is not applicable due to spider's fierce behavior. The current approach is to produce recombinant spidroins (silk proteins) from other chassis and spin them into silk. This year , we aim to manufacture recombinant spider silk with E.coli and color the silk for application in cloth industry. We modularized three significant domains of spidroin - the N-terminal, the repetitive region, and the C-terminal - and integrated them into various spidroin to form silk. We then dyed the silk with microbial natural pigments deoxyviolacein and indigo. To obtain better color and a more convenient dying process, we fused the repetitive region to chromoproteins and mixed them with spidroin during spinning. Our team hopes to provide a novel approach for cloth production and explore new possibilities for spider silk applications.

3. 隊名：Mingdao

項目名稱：Fresh Air, the clever heir

獎項：

（1）Best Education and Public Engagement;
（2）Best Integrated Human Practices;
（3）Best Model;
（4）Best New Basic Part;
（5）Best New Composite Part;
（6）Best Part Collection;
（7）Best Poster;
（8）Best Supporting Entrepreneurship;
（9）Best Wiki

摘要/項目介紹：

To increase our algae system's efficiency of reducing indoor pollutants such as CO2 and VOCs, we applied two enzymes, carbonic anhydrase(CA) and cytochrome P450 2E1(CYP2E1) to improve algal absorption of CO2 and VOCs, respectively. We produced enzymes by engineering Bacillus subtilis 168 with BioBricks we created and then finally put them into our system. We also did some experiments to analyze the activity of our enzymes and test whether our system is efficient enough to refresh indoor air.

4. 隊名：GENAS_China

項目名稱：Recombinase-Based Biological Relay devices (RBBR),

獎項：

（1）Best Measuremen;
（2）Best Model;
（3）Best New Basic Part;
（4）Best Part Collection;Best Wiki

摘要/獎項介紹：

Generally, the relay, the key component of electrical automatic control system, receives the output signal of a control module and thus shifts the ON/OFF state of a separated working module. Based on the recombinase-attB/attP system and unidirectional terminator, we constructed a set of orthogonal Recombinase-Based Biological Relay devices (RBBR), whose response intervals were characterized by accurate quantifying method so that they can be predictably adapted to different genetic circuits. We designed and constructed a resolution extensible analog-digital converter (ADC), which converts the consecutive analog quantities (the strength of an inducible promoter) into discrete digital signals (indicated by different chromoproteins), allowing the digitized processing and storage of signals. Beyond the common use of recombinase system as simple response to two input levels, our project achieves modifying and utilizing the response interval of this system. The application of relay in genetic circuit can contribute to the improvement of the modularity of artificial biological system.

5. 隊名：US_AFRL_CarrollHS

項目名稱：ENGINEERING A CELL-FREE HEXAVALENT CHROMIUM DETECTION SYSTEM

獎項：Best Education and Public Engagement

摘要/項目介紹：

Methylophaga flavin-containing monooxygenase (bFMO) is the gene responsible for the conversion of the Indole into Isatin, which is then catalyzed into indigoid compounds. These compounds display an indigo color which can be easily detected (KAIST). Our project will focus on using bFMO as indicator to determine when toxic hexavalent chromium is present. The bFMO will be cloned into the C4-HSL circuit created by our team last year, in order to show the indigo color can be produced and easily seen. This will be done by replacing GFPa1 (green fluorescent protein) with the bFMO gene. Next, bFMO will be cloned into a construct with a T7 promoter, which we believe will be the most efficient promoter for bFMO. This construct will then be tested to ensure that the promoter will produce bFMO. Once this is done, the chrB repressor will be introduced. In order to determine when hexavalent chromium is present, a repressor, chrB, will be cloned into a construct along with the promoter, chrP, associated with the repressor. This construct will then be cloned into a pet11a backbone, which already has both the T7 promoter and rbs needed. The plasmid should then produce chrB, which will disrupt the chrP causing bFMO to not be produced. A commercial cell-free extract kit will then be used on this construct, and the T7-chrB plasmid will be added in order to make ChrB. When chromium is detected the ChrB repressor will be ‘knocked off,’ allowing the promoter to function and bFMO to catalyze into indigoid compounds, emitting a detectable indigo color.

6. 隊名：SHSBNU_China

項目名稱：/

獎項：Best New Composite Part

摘要/項目介紹：

DNA is a biological macro-molecule which can carry huge amount of information accurately, and this feature can be used to achieve data recordings in vivo. Our project aims to build a biological recorder that can monitor extracellular information and record it on DNA. Recombinase or CRISPR base-editor is used to target specific DNA addresses and generate mutations in a reporter gene, so the recordings can be quantitatively measured to infer intensity and duration information about the chemicals of disease, such as inflammatory bowel disease. Additionally, we designed a hardware for in situ biomolecular detection to monitor gastrointestinal health. This platform could enable more precise detection and could help improve the management and diagnosis of gastrointestinal disease.

7. 隊名：Greatbay_SCIE

項目名稱：Stickit

獎項：Best Part Collection

摘要/項目介紹：

To create a well-functioning adhesive, cohesion--the ability to maintain the shape of the material--and adhesion--the ability to stick to underwater surfaces--should cooperate. Our toolbox consists of several groups of biological parts from marine creatures (mussel and barnacles) and prokaryotes (E.coli MG1655 and Marine Archaea). These proteins will provide cohesion, adhesion, and other special qualities. We will then combine these proteins using principles of synthetic biology to create protein adhesives with high-performance and biocompatibility. Their potentials are limitless and can be applied to many fields.

8. 隊名：TAS_Taipei

項目名稱：ADIEU, RESIDUES!

獎項：Best Part Collection

摘要/項目介紹：

We consume fruits and vegetables every day without knowing whether or not agricultural residues (i.e. pesticides & heavy metals) are present. In 2017, according to the UN, exposure to pesticides caused 200,000 to 300,000 deaths annually. Additionally, lead exposure alone is responsible for a death rate of 25.3 per 100,000 individuals in East Asia. Current methods of agricultural residue detection are not easily accessible to the public and are inconvenient for everyday use. Thus, our project aims to allow for convenient visualization of agricultural residues by designing colored proteins that can directly interact with these residues. We envision a system where our designed proteins can be applied directly on food items to detect the presence of residues. Our final product can be used by consumers, distributors and farmers alike.

9. 隊名：TPHS_San_Diego

項目名稱：Breakdown of Trimethylamine via Trimethylamine Dehydrogenase to Minimize Heart Disease Caused by Red Meat Consumption

獎項：Best Plant Synthetic Biology

摘要/項目介紹：

The consumption of red meat has been linked to atherosclerosis, a form of heart disease caused by the buildup of plaque in the arteries. Recently, it was discovered that the combination of choline and L-carnitine from red meat, are converted to trimethylamine (TMA) in the body. TMA is a precursor to trimethylamine N-oxide (TMAO), which is found to exacerbate cholesterol buildup, ultimately leading to atherosclerosis. In this study, we targeted this pathway by breaking down the precursor, TMA, before TMAO is formed. A system designed to concurrently degrade TMA and subdue its toxic by-product, formaldehyde, was implemented in E. coli for these purposes. This system is composed of Trimethylamine Dehydrogenase (TMADH) and Formaldehyde Dehydrogenase (FDH) which are being tested independently for their degradation properties. Our approach has proven that TMADH is effective in TMA degradation and FDH can be expressed in a bacterial vector to minimize the presence of formaldehyde.

師資團(tuán)隊（部分）

師資團(tuán)隊由合成生物學(xué)領(lǐng)域國際知名科學(xué)家 、杰出青年學(xué)者以及經(jīng)驗豐富的一線課程設(shè)計師、講師組成，兼具專業(yè)的科學(xué)素養(yǎng)與教育素養(yǎng)。

宗夜晴

中國科學(xué)院微生物研究所博士

具有豐富的合成生物學(xué)研究經(jīng)驗，曾承擔(dān)多個研發(fā)項目，發(fā)表高影響因子論文數(shù)篇；

孟凡康

中國科學(xué)院微生物研究所在讀博士

合成生物學(xué)知名自媒體《再創(chuàng)》主編，原南開大學(xué)iGEM金獎隊伍核心成員。

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