Compartmentalized cell structure

• Eukaryotic cells have a more?complex ultrastructure?than prokaryotic cells
• The cytoplasm of eukaryotic cells is divided up into?membrane-bound?compartments called?organelles. These compartments are either bound by a?single?or?double membrane
• The?compartmentalization?of the cell is?advantageous?as it allows:
  • Enzymes and substrates to be localised and therefore available at higher concentrations
  • Damaging substances to be kept separated, e.g. digestive enzymes are stored in lysosomes so they do not digest the cell
  • Optimal conditions to be maintained for certain processes e.g. optimal pH for digestive enzymes
  • The numbers and location of organelles to be altered depending on requirements of the cell
• Eukaryotic?cells have a key compartment called the?nucleus

Animal and plant cells

• Animal and plant cells are both types of eukaryotic cells that share key structures such as:
  • Membrane-bound organelles, including a nucleus
  • Larger ribosomes (80S)
• However, there are key differences:
  • Animal cells contain?centrioles?and?microvilli
  • Plant cells have a cellulose?cell wall,?large permanent?vacuoles?and?chloroplast

The ultrastructure of an animal cell shows a densely packed cell – the ER and RER and ribosomes form extensive networks throughout the cell in reality

Plant cells have a larger, more regular structure in comparison to animal cells

• In complex?multicellular?organisms,?eukaryotic?cells become?specialised?for?specific functions
• These specialised eukaryotic cells have?specific adaptations?to help them carry out their functions
• For example, the?structure of a cell?is adapted to help it carry out its?function?(this is why specialised eukaryotic cells can look extremely?different?from each other)
• Structural adaptations include:
  • The?shape?of the cell
  • The?organelles?the cell contains (or doesn’t contain)
• For example:
  • Red blood cells are?biconcave?and?do not contain a nucleus.?This makes?more space?inside the cell so that they can transport as much?oxygen?as possible
  • Cells that make large amounts of?proteins?will be adapted for this function by containing?many ribosomes?(the organelle responsible for protein production)

Organelles

Plasma membrane

The structure of the cell surface membrane – although the structure looks static the phospholipids and proteins forming the bilayer are constantly in motion

• All cells?are surrounded by a plasma membrane which controls the exchange of materials between the internal cell environment and the external environment
  • The membrane is described as being ‘partially permeable’
• The plasma membrane is formed from a?phospholipid bilayer?of phospholipids spanning a diameter of around 10 nm

Nucleus

The nucleus of a cell contains chromatin (a complex of DNA and histone proteins) which is the genetic material of the cell

• Present?in all eukaryotic cells?(except red blood cells), the nucleus is relatively large and separated from the cytoplasm by a double membrane (the?nuclear envelope)?which has many pores
• Nuclear pores are important channels for allowing mRNA and ribosomes to travel out of the nucleus, as well as allowing enzymes (eg. DNA polymerases) and signalling molecules to travel in
• The nucleus contains?chromatin?(the material from which chromosomes are made)
  • Chromosomes are made of sections of?linear DNA?tightly wound around proteins called?histones
• Usually, at least one or more darkly stained regions can be observed – these regions are individually termed ‘nucleolus’ (plural: nucleoli) and are the sites of?ribosome production

Rough endoplasmic reticulum

The rough endoplasmic reticulum (RER) - the attached ribosomes enable this structure to be identified in electron micrographs

• Found in plant and animal cells
• Surface covered in?ribosomes?(80S)
• Formed from continuous folds of membrane continuous with the?nuclear envelope. These flattened membrane sacs are called?cisternae
• Processes proteins made by the?ribosomes
• The?proteins?synthesised by the ribosomes, move to the cisternae, bud off into vesicles that carry the proteins to Golgi apparatus before being?secreted out?of the cell

Ribosomes

Ribosomes are formed in the nucleolus and are composed of almost equal amounts of RNA and protein

• Found freely in the cytoplasm of?all cells?or as part of the?rough endoplasmic reticulum?in eukaryotic cells
• Each ribosome is a complex of?ribosomal RNA (rRNA)?and proteins. They are constructed in the nucleolus (a region in the nucleus)
• 80S ribosomes?(composed of 60S and 40S subunits) are found in eukaryotic cells
• Site of translation (protein synthesis)

Mitochondrion

A single mitochondrion is shown – the inner membrane has protein complexes vital for the later stages of aerobic respiration embedded within it

• The site of aerobic respiration within?all eukaryotic cells, mitochondria are just visible with a light microscope
• Surrounded by?double-membrane?with the inner membrane folded to form?cristae
• The matrix formed by the cristae contains enzymes needed for?aerobic respiration,?producing?ATP
• Small circular pieces of?DNA?(mitochondrial DNA) and ribosomes are also found in the matrix (needed for replication)

Golgi apparatus

The structure of the Golgi apparatus

• Found in plant and animal cells
• Flattened sacs of membrane called cisternae (like the rough endoplasmic reticulum)
• Modifies?proteins and lipids before?packaging?them into?Golgi vesicles
  • The vesicles then?transport the proteins and lipids?to their required destination
  • Proteins that go through the Golgi apparatus are usually exported (e.g. hormones such as insulin), put into lysosomes (such as hydrolytic enzymes) or delivered to membrane-bound organelles

Vesicles

The structure of the vesicle

• Found in plant and animal cells
• A membrane-bound sac for transport and storage

Lysosome

The structure of the lysosome

• Specialist forms of vesicles which contain?hydrolytic enzymes?(enzymes that break biological molecules down)
• Break down waste materials such as worn-out organelles
• Used extensively by cells of the?immune system?and in?apoptosis?(programmed cell death)

Chloroplasts

Chloroplasts are found in the green parts of a plant – the green colour a result of the photosynthetic pigment chlorophyll

• Found in?plant cells
• Larger than mitochondria
• Surrounded by a?double-membrane
• Membrane-bound compartments called?thylakoids?containing?chlorophyll?stack to form structures called?grana
• Grana are joined together by?lamellae?(thin and flat thylakoid membranes)
• Chloroplasts are the site of?photosynthesis:
  • The?light-dependent stage?takes place in the thylakoids
  • The?light-independent stage?(Calvin Cycle) takes place in the?stroma
• Also contain small circular pieces of?DNA?and ribosomes used to synthesise proteins needed in chloroplast replication and photosynthesis

Large permanent vacuoles

The structure of the vacuole

• A sac in?plant cells?surrounded by the?tonoplast, selectively permeable membrane
• Vacuoles in animal cells are not permanent and small

Cell wall - an extra-cellular component (not an organelle)

The cell wall is freely permeable to most substances (unlike the plasma membrane)

• Found in plant cells but?not in animal cells
• Cell walls are formed outside of the cell membrane and offer?structural support?to cell
• Structural support is provided by the polysaccharide cellulose in plants, and peptidoglycan in most bacterial cells
• Narrow threads of cytoplasm (surrounded by a cell membrane) called?plasmodesmata?connect the cytoplasm of neighbouring plant cells

Additional organelles

• The below organelles can be found in other specialised cells in eukaryotes

Flagella

The structure of the flagella

• Found in specialised cells
• Similar in structure to?cilia, made of longer?microtubules
• Contract to provide cell movement for example in?sperm cells

Centrioles

The structure of the centriole

• Hollow fibres made of?microtubules
• Two centrioles at right angles to each other form a?centrosome, which organises the?spindle fibres?during cell division
• Not found?in?flowering plants?and?fungi

Microtubules

The structure of the microtubule

• Found in all eukaryotic cells
• Makes up the cytoskeleton of the cell about 25 nm in diameter
• Made of α and β tubulin combined to form dimers, the dimers are then joined into protofilaments
  • Thirteen protofilaments in a cylinder make a microtubule
• The cytoskeleton is used to provide support and movement of the cell

Microvilli

The structure of the microvilli

• Found in specialised animal cells
• Cell membrane projections
• Used to?increase the surface area?of the cell surface membrane in order to increase the rate of exchange of substances

Cilia

The structure of the cilia

• Hair-like projections made from?microtubules
• Allows the movement of substances over the cell surface