It is important to use?appropriate apparatus?when recording a range of quantitative measurements
Quantitative measurements include things such as mass, time, volume, temperature, length and pH
Mass?should be recorded using a?digital balance
Time?should be recorded using a?digital stopwatch
Volume?should be recorded using a?measuring cylinder
Temperature?should be recorded using a?digital thermometer?(although water baths have one built-in)
Length?should be recorded using a?ruler
The practical below is an example of an experiment that has used the correct apparatus to measure the?dependent variable
Practical: Investigating the rate of diffusion using agar
The effect of?surface area to volume ratio?on the?rate of diffusion?can be investigated by?timing?the diffusion of ions through?different sized cubes of agar
Coloured agar is cut into cubes of the required dimensions (eg. 0.5cm x 0.5cm x 0.5cm, 1cm x 1cm x 1cm and 2cm x 2cm x 2cm)
Purple agar can be created if it is made up with very dilute sodium hydroxide solution and Universal Indicator
Alternatively, the agar can be made up with Universal Indicator only
The cubes are then placed into boiling tubes containing a diffusion solution (such as dilute hydrochloric acid)
The acid should have a higher molarity than the sodium hydroxide so that its diffusion can be monitored by a change in colour of the indicator in the agar blocks
Measurements can be taken of either:
The?time taken?for the acid to?completely?change?the?colour?of the?indicator?in the agar blocks
A?stopwatch?is used
The?distance travelled?into the block by the acid (shown by the change in colour of the indicator) in a?given time period?(eg. 5 minutes)
A?microscope and a stage micrometer?(essentially a mini ruler) are used
These times can be converted to rates (1 ÷ time taken)
A?graph?could be drawn showing how the?rate?of diffusion (rate of colour change)?changes?with the?surface area to volume ratio?of the agar cubes
An example of how to set up an experiment to investigate the effect of changing surface area to volume ratio on the rate of diffusion
Exam Tip
When an agar cube (or for example a biological cell or organism) increases in size, the volume increases faster than the surface area, because the volume is cubed whereas the surface area is squared. When an agar cube (or biological cell / organism) has more volume but proportionately less surface area, diffusion takes longer and is less effective. In more precise scientific terms, the?greater the surface area to volume ratio, the?faster the rate of diffusion!
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